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. 2018 Aug 24;16(8):e2002979. doi: 10.1371/journal.pbio.2002979

Fig 2. FACS dot plot of M-CSFRGD affinity maturation process.

Fig 2

Yeast-displayed mutant pools were tested for binding to (A) 200 nM c-FMS, (B) 500 nM αvβ3 integrin, (C) 250 nM αvβ3 integrin, (D) 100 nM αvβ3 integrin, (E) 20 nM αvβ3 integrin, and (F) 50 nM c-FMS. High target binders were sorted as indicated in each figure with black square- or polygon-shaped gates. M-CSF, macrophage colony-stimulating factor; RGD, Arginine-Glycine-Aspartic acid.