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. 2018 Aug 24;16(8):e2004506. doi: 10.1371/journal.pbio.2004506

Fig 1. prd1 is required for dendrite pruning in sensory neurons.

Fig 1

(A) A schematic representation of dendrite pruning and regrowth in ddaC neurons during early metamorphosis. Soma and axon are depicted in pink while dendrites are in black. (B–H) Dendrites of control (B), prd1 RNAi #1 (C), prd1M56/Df(3R)Exel7310 (D), prd1M56 rescue using full-length Prd1 (E), or Venus-Prd1 (F), prd1PS1 MARCM (G) and prd1PS1 MARCM rescue using full-length Prd1 (H) ddaC neurons at WP and 16 h APF. Red arrowheads point to the ddaC somas. (I) Quantification of severing defect and fragmentation defect in control and mutant ddaC neurons at 16 h APF. (J) Quantification of total length of unpruned ddaC dendrites at 16 h APF. The number of samples (n) in each group is shown on the bars. Error bars represent SEM. Scale bar in (B) represents 50 μm. ***p < 0.001 as assessed by one-way ANOVA test. The individual numerical values for panels I and J can be found in S1 Data. The genotypes can be found in S1 Text. APF, after puparium formation; MARCM, mosaic analysis with a repressible cell marker; n.s., not significant; O/E, overexpression; prd1, pruning defect 1; RNAi, RNA interference; WP, white prepupal.