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. 2018 Aug 24;14(8):e1007287. doi: 10.1371/journal.ppat.1007287

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© 2018 Wang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — (A) Confocal microscopy of TRIM69-Myc (red) and NS3-Flag or NS4B-Flag (green). NS3-Flag or NS4B-Flag was transfected into Hela cells with or without TRIM69-Myc for 48 h, and then the cells were treated with MG132 for 4 h before fixation. The nuclear was stained with DAPI. (B) Co-IP of lysates from 293T cells expressed TRIM69-Myc with or without NS3-Flag. IP was performed using Flag antibody. (C) Reciprocal Co-IP of lysates from 293T cells transfected with NS3-Flag with or without TRIM69-Myc. IP was performed using Myc antibody. (D, E) Co-IP of endogenous TRIM69 and NS3 from lysates of 293T cells infected with DENV-2 for 48 h. IP was performed with TRIM69 antibody (D) or NS3 antibody (E). IgG was used as a control. (F) GST-pulldown assays of GST-NS3 with purified TRIM69-Myc. Representative blots from three different repeats were shown.