Table 2. Plasmids used in this study.
Plasmid | Description | Source | Figure |
---|---|---|---|
Ycplac33 | CEN, URA3 | [79] | |
WCC6 | [Ycplac33] prWHI2-WHI2 | [19] | 1D |
BQ23 | 2μ, URA3, prPGK1 | This study | 1G; 4; 5G, 5H; 6C–6F; 7A |
TXC19 | [BQ23] prPGK1-HA-WHI2 | This study | 1G; 4; 5G, 5H; 6C–6F; 7A |
CXH4 | [BQ23] prPGK1-HA-NPR2 | This study | 4B–4D |
CXH5 | [BQ23] prPGK1-HA-NPR3 | This study | 4B, 4C, 4E |
RS416 | CEN, URA3 | [80] | |
p2285 | [pRS416] prADH1-IML1-HIS6-TEV-ProtA | [24] | 4A and 4C |
BQ8 | 2μ, URA3, prPGK1 | This study | 5A |
Gtr1 | [BQ8] prPGK1-GTR1 | This study | 5A–5F |
Gtr1GDP | [BQ8] prPGK1-GTR1-S20L | This study | 5A and 5B |
Gtr1GTP | [BQ8] prPGK1-GTR1-Q65L | This study | 5A–5F |
RS313 | CEN, HIS3 | [81] | 5E and 5F |
ZY1 | [pRS313] prPGK1-HA-WHI2 | This study | 5E and 5F |
WGQ1 | [BQ23] prPGK1-HA-PSR1 | This study | 6C–6H |
WGQ2 | [BQ23] prPGK1-HA-PSR2 | This study | 6C–6E |
ZMJ11 | [pRS313] prPGK1-3xFLAG-WHI2 | This study | 6H |
TXC20 | [BQ23] prPGK1-HA-hKCTD7 | This study | 7A |
TXC21 | [BQ23] prPGK1-HA-mKCTD8 | This study | 7A |
TXC33 | [BQ23] prPGK1-HA-hKCTD11 | This study | 7A |
DB59 | prSV40, N-HA | This study | 7B and 7C |
HA-hKCTD7 | [DB59] prSV40-HA-hKCTD7 | This study | 7B |
HA-hKCTD12 | [DB59] prSV40-HA-hKCTD12 | This study | 7B |
HA-hKCTD16 | [DB59] prSV40-HA-hKCTD16 | This study | 7B |
FLAG-mKCTD8 | [pCI] prCMV-FLAG-mKctd8 | [15] | 7B |
HA-hKCTD11 | [DB59] prSV40-HA-hKCTD11 | This study | 7C, 7E; S5A and S5B |
WGQ4 | [Ycplac33] prDAL80-GFP | This study | S3A |
AS103 | [YEplac195] prNPR1-HA-NPR1 | [45] | S3B |
ZMJ2 | [pRS313] prPGK1-HA-PDE2 | This study | S4C and S4D |
WGQ5 | [pRS313] prDAL80-GFP | This study | S5A |