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. Author manuscript; available in PMC: 2018 Nov 9.
Published in final edited form as: Nature. 2018 May 9;557(7705):434–438. doi: 10.1038/s41586-018-0092-4

Extended Data Figure 5. Minimal porphyran utilization PULs were constructed via PCR and yeast assembly.

Extended Data Figure 5

Schematic representing construction of designed porphyran PULs. Based on alignment to previously published B. plebeius porphyran utilization PUL, three regions were targeted for minimal PUL assembly and amplified via PCR from the NB001 genome. The PCR fragments were assembled with digests of both a custom and commercially available vector in yeast (see Methods), after which colonies carrying correctly assembled plasmids were lysed and directly applied to E. coli for electroporation.