Fig. 4.

O-GlcNAcylation regulates the half-life of Nod1. (A) HEK293T-Nod1-expressing cells were incubated with 1 μM Thiamet-G or DMSO for 4 h, prior to cycloheximide treatment (50 μg/mL) and cells were collected at the indicated time intervals. Nod1 was detected by anti-Flag western blotting. (B) Half-lives were determined by plotting relative Flag intensities vs. time assuming first order decay, and calculating T_1/2 = ln(2)/k