Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M.musculus) |
Fermt3-/- | PMID: 18278053 | RRID: MGI:2147790 | Dr. Reinhard Fässler (Max Planck Institute of Biochemistry) |
| Genetic reagent (M.musculus) |
Fermt3flox | PMID: 24089451 | RRID: MGI:5551978 | Dr. Reinhard Fässler (Max Planck Institute of Biochemistry) |
| Genetic reagent (M.musculus) |
Fermt3n/- | PMID: 26438512 | RRID: MGI:3785479 | Dr. Reinhard Fässler (Max Planck Institute of Biochemistry) |
| Genetic reagent (M.musculus) |
Rag2-/- | PMID: 1547487 | RRID: MGI:1858556 | Dr. Frederick W Alt (Howard Hughes Medical Institute) |
| Genetic reagent (M.musculus) |
Mx1-Cre | PMID: 7660125 | RRID: MGI:2176073 | Dr. Klaus Rajewsky (Max Delbrück Center for Molecular Medicine) |
| Genetic reagent (M.musculus) |
CD4-Cre | PMID: 11728338 | RRID: MGI: 2386448 | Dr. Christopher B. Wilson, (University of Washington) |
| Genetic reagent (M.musculus) |
2D2 | PMID: 12732654 | RRID: MGI:3700794 | Dr. Vijay K Kuchroo (Center for Neurologic Diseases) |
| Genetic reagent (M.musculus) |
mTmG | PMID: 17868096 | RRID: MGI:3716464 | Dr. Liqun Luo (Howard Hughes Medical Institute) |
| Antibody | anti-B220-biotin | BD Pharmingen | Cat. #: 553085; RRID: AB_394615 |
FACS (1:200) |
| Antibody | POD-coupled anti-BrdU |
Roche | Cat. #: 11 585 860 001; RRID: AB_514485 |
IHC (1:30) |
| Antibody | rabbit anti-mouse cleaved caspase-3 |
Cell Signalling | Cat. #: 9661; RRID: AB_2341188 |
IHC (1:100) |
| Antibody | anti-CD3e | eBioscience | Cat. #: 14-0031-82; RRID: AB_467049 |
10 µg/ml |
| Antibody | anti-CD3e-biotin | BD Pharmingen | Cat. #: 553060; RRID: AB_394593 |
FACS (1:200) |
| Antibody | anti-CD3-PE | BD Pharmingen | Cat. #: 555275; RRID: AB_395699 |
FACS (1:200) |
| Antibody | anti-CD4-biotin | BD Pharmingen | Cat. #: 553649; RRID: AB_394969 |
FACS (1:200) |
| Antibody | anti-CD4-FITC | BD Pharmingen | Cat. #: 553651; RRID: AB_394971 |
FACS (1:200) |
| Antibody | anti-CD4-PerCP | BD Pharmingen | Cat. #: 550954; RRID: AB_393977 |
FACS (1:200) |
| Antibody | anti-CD5-PE | eBioscience | Cat. #: 12-0051-82; RRID: AB_465523 |
FACS (1:200) |
| Antibody | anti-CD8α-biotin | BD Pharmingen | Cat. #: 553028; RRID: AB_394566 |
FACS (1:200) |
| Antibody | anti-CD8-APC | eBioscience | Cat. #: 17-0081-81; RRID: AB_469334 |
FACS (1:200) |
| Antibody | anti-CD8-PE | eBioscience | Cat. #: 12-0081-81; RRID: AB_465529 |
FACS (1:200) |
| Antibody | anti-CD11b-biotin | BD Pharmingen | Cat. #: 557395; RRID: AB_2296385 |
FACS (1:200) |
| Antibody | anti-CD11c-biotin | BD Pharmingen | Cat. #: 553800; RRID: AB_395059 |
FACS (1:200) |
| Antibody | anti-CD16/CD32 | BD Pharmingen | Cat. #: 553142; RRID:AB_394657 |
FACS (1:400) |
| Antibody | anti-CD19-biotin | eBioscience | Cat. #: 13-0193-82; RRID: AB_657656 |
FACS (1:200) |
| Antibody | anti-CD24-PE | BD Pharmingen | Cat. #: 553262; RRID: AB_394741 |
FACS (1:200) |
| Antibody | anti-CD25-FITC | BD Pharmingen | Cat. #: 553071; RRID: AB_394603 |
FACS (1:200) |
| Antibody | anti-CD28 | eBioscience | Cat. #: 14-0281-82; RRID: AB_467190 |
2 µg/ml |
| Antibody | rat anti-CD31 (PECAM-1) | BD Pharmingen | Cat. #: 557355; RRID: AB_396660 |
IHC (1:300) |
| Antibody | anti-CD31- Alexa Fluor 647 |
Biolegend | Cat. #: 102415; RRID: AB_493411 |
IHC (1:200) |
| Antibody | anti-CD31-eFLour450 | eBioscience | Cat. #: 48-0311-82; RRID: AB_10598807 |
FACS (1:200) |
| Antibody | anti-CD44-PE | BD Pharmingen | Cat. #: 553134; RRID: AB_394649 |
FACS (1:200) |
| Antibody | anti-CD45-FITC | eBioscience | Cat. #: 14-0451-82; RRID: AB_467251 |
IHC (1:100) |
| Antibody | anti-CD45.2-biotin | BD Pharmingen | Cat. #: 553771; RRID: AB_395040 |
FACS (1:200) |
| Antibody | anti-CD45.2-APC | eBioscience | Cat. #: 17-0454-82; RRID: AB_469400 |
FACS (1:200) |
| Antibody | hamster anti- CD54 (ICAM) |
BD Pharmingen | Cat. #: 553250; RRID: AB_394732 |
IHC (1:100) |
| Antibody | goat anti- CD106 (VCAM) |
R and D Systems | Cat. #: AF643; RRID: AB_355499 |
IHC (1:80) |
| Antibody | anti-c-kit-APC | eBioscience | Cat. #: 17-1171-81; RRID: AB_469429 |
FACS (1:200) |
| Antibody | anti-c-kit-PerCP | Biolegend | Cat. #: 105821; RRID: AB_893230 |
FACS (1:200) |
| Antibody | mouse anti-pan -cytokeratin |
Sigma-Aldrich | Cat. #: C2562; RRID: AB_476839 |
IHC (1:400) |
| Antibody | anti-EpCAM-APC -eFlour780 |
Thermo Fisher Scientific | Cat. #: 47-5791-82; RRID: AB_2573986 |
FACS (1:200) |
| Antibody | rat anti-ER-TR4 | Eric Vroegindeweij (Princess Maxima center of pediatric oncology) |
IHC undiluted | |
| Antibody | rat anti-ER-TR5 | Eric Vroegindeweij (Princess Maxima center of pediatric oncology) |
IHC undiluted | |
| Antibody | anti-F4/80-biotin | Bio-Rad | Cat. #: MCA497GA; RRID: AB_323806 |
FACS (1:200) |
| Antibody | alexa-fluor-488- conjugated anti- Fluorescin IgG |
Thermo Fisher Scientific |
Cat. #: A-11090; RRID: AB_221562 |
IHC (1:500) |
| Antibody | rat anti-ICAM-1 PE | Biolegend | Cat. #:116107; RRID: AB_313698 |
FACS (1:200) |
| Antibody | rabbit anti-Fibronectin | Merck Millipore | Cat. #: AB2033; RRID: AB_2105702 |
IHC (1:300) |
| Antibody | mouse anti-GAPDH | Merck Millipore | Cat. #: CB1001; RRID: AB_2107426 |
WB (1:20.000) |
| Antibody | anti-Gr-1-biotin | BD Pharmingen | Cat. #: 553125; RRID: AB_394641 |
FACS (1:200) |
| Antibody | alexa-fluor-546 -conjugated anti-goat IgG |
Thermo Fisher Scientific |
Cat: #: A-11056; RRID: AB_2534103 |
IHC (1:300) |
| Antibody | alexa-fluor-546- conjugated anti-hamster IgG |
Thermo Fisher Scientific |
Cat: #: A-21111; RRID: AB_2535760 |
IHC (1:300) |
| Antibody | alexa-fluor-546- conjugated anti-mouse IgG |
Thermo Fisher Scientific |
Cat: #: A-11003; RRID: AB_2534071 |
IHC (1:300) |
| Antibody | alexa-fluor-647 -conjugated anti-mouse IgG |
Thermo Fisher Scientific |
Cat: #: A-31571; RRID: AB_162542 |
IHC (1:300) |
| Antibody | alexa-fluor-647 -conjugated anti-rabbit IgG |
Thermo Fisher Scientific |
Cat: #: A-21244; RRID: AB_2535812 |
IHC (1:300) |
| Antibody | alexa-fluor-488- conjugated anti-rat IgG |
Thermo Fisher Scientific |
Cat: #: A-11006; RRID: AB_2534074 |
IHC (1:300) |
| Antibody | alexa-fluor-647- conjugated anti-rat IgG |
Thermo Fisher Scientific |
Cat: #: A-21247; RRID: AB_141778 |
IHC (1:300) |
| Antibody | anti-Integrin α4-PE | BD Pharmingen | Cat. #: 553157; RRID: AB_394670 |
FACS (1:200) |
| Antibody | anti-Integrin αL-PE | BD Pharmingen | Cat. #: 553121; RRID: AB_394637 |
FACS (1:200) |
| Antibody | anti-Integrin β1-PE | Biolegend | Cat. #: 102207; RRID: AB_312884 |
FACS (1:200) |
| Antibody | anti-Integrin β2-PE | BD Pharmingen | Cat. #: 553293; RRID: AB_394762 |
FACS (1:200) |
| Antibody | anti-Integrin β3-PE | eBioscience | Cat. #: 12-0611-81; RRID: AB_465717 |
FACS (1:200) |
| Antibody | anti-Integrin β7-PE | BD Pharmingen | Cat. #: 557498; RRID: AB_396735 |
FACS (1:200) |
| Antibody | rabbit anti-Kindlin-1 | Markus Moser (MPI of Biochemistry) |
WB (1:1000) | |
| Antibody | mouse anti-Kindlin-2 | Sigma-Aldrich | Cat. # SAB4200525 -200UL |
WB (1:1000) |
| Antibody | rabbit anti-Kindlin-3 | Markus Moser (MPI of Biochemistry) |
WB (1:3000) | |
| Antibody | rabbit anti-pan-laminin |
Rupert Timpl | IHC (1:700) | |
| Antibody | anti-LFA-1-PE | BD Pharmingen | Cat. #: 553121; RRID: AB_394637 |
IF (1:100) |
| Antibody | anti-NK1.1-biotin | BD Pharmingen | Cat. #: 553163; RRID: AB_394675 |
FACS (1:200) |
| Antibody | anti-PIR-APC | eBioscience | Cat. #: 17-3101-82; RRID: AB_1944406 |
FACS (1:200) |
| Antibody | anti-PIR-PE | BD Pharmingen | Cat. #: 550349; RRID: AB_393628 |
FACS (1:200) |
| Antibody | anti-p-Tyrosine | Santa Cruz Biotechnology |
Cat. #: sc-7020; RRID: AB_628123 |
IF (1:50) |
| Antibody | anti-Sca-1-PE | eBioscience | Cat. #: 12-5981-82; RRID: AB_466086 |
FACS (1:200) |
| Antibody | mouse anti-talin | Sigma-Aldrich | Cat. #: T3287; RRID: AB_477572 |
WB (1:20.000) |
| Antibody | anti-TCRβ-PE | eBioscience | Cat. #: 12-5961-82; RRID: AB_466066 |
FACS (1:200) |
| Antibody | anti-TCRγδ-biotin | BD Pharmingen | Cat. #: 553176; RRID: AB_394687 |
FACS (1:200) |
| Antibody | anti-Ter119-biotin | BD Pharmingen | Cat. #: 553672; RRID: AB_394985 |
FACS (1:200) |
| Antibody | anti-Thy1.2-biotin | BD Pharmingen | Cat. #: 553001; RRID: AB_394540 |
FACS (1:200) |
| Antibody | rat anti-VCAM-1 PE | Biolegend | Cat. #: 105713; RRID: AB_1134166 |
FACS (1:200) |
| Peptide, recombinant protein |
recombinant mouse CCL21 |
R and D Systems | Cat. #: 457–6C; Accession #: P84444.1 |
|
| Peptide, recombinant protein |
recombinant mouse CCL25 |
R and D Systems | Cat. #: 481-TK; Accession #: O35903.1 |
|
| Peptide, recombinant protein |
recombinant mouse ICAM-1-FC |
R and D Systems | Cat. #: 796-IC; Accession #: Q3U8M7 |
|
| Peptide, recombinant protein |
MOG35-55 | MPI of Biochemistry, Core facility |
||
| Peptide, recombinant protein |
recombinant mouse P-Selectin-FC |
R and D Systems | Cat. #: 737-PS; Accession #: Q01102 |
|
| Peptide, recombinant protein |
recombinant mouse VCAM-1-FC |
R and D Systems | Cat. #: 643-VM; Accession #: CAA47989 |
|
| Commercial assay or kit |
PE Annexin V Apoptosis Detection Kit |
BD Biosciences | Cat. #: 559763 | |
| Commercial assay or kit |
BrdU Flow Kit | BD Pharmingen | Cat. #: 559619; RRID: AB_2617060 |
|
| Commercial assay or kit |
anti-biotin MicroBeads |
Miltenyi | Cat. #: 130-090-485; RRID: AB_244365 |
|
| Commercial assay or kit |
CD4 T cell isolation kit |
Miltenyi | Cat. #: 130-104-454 | |
| Commercial assay or kit |
Vectastain Elite ABC HRP Kit, rabbit IgG |
VECTOR laboratories |
Cat. #: PK-6101; RRID: AB_2336820 |
|
| Chemical compound, drug |
CellTrace CFSE Cell Proliferation Kit |
Thermo Fisher Scientific |
Cat. #: C34554 | |
| Chemical compound, drug |
Collagenase D | Sigma-Aldrich | Cat. #: C0130 | 1 mg/ml |
| Chemical compound, drug |
FITC-Dextran | Sigma-Aldrich | Cat. #: FD150S | |
| Chemical compound, drug |
DNaseI | Sigma-Aldrich | Cat. #: D5025 | 100 U/ml |
| Chemical compound, drug |
dGuo | Sigma-Aldrich | Cat. #: D0901 | 1.35 mM |
| Chemical compound, drug |
CellTrace Far Red Cell Proliferation Kit |
Thermo Fisher Scientific |
Cat. #: C34564 | 1 µM |
| Chemical compound, drug |
FluoSpheres 580/606 | Thermo Fisher Scientific |
Cat. #: F8821 | 1 µM |
| Chemical compound, drug |
LPS | Sigma-Aldrich | Cat. #: L2654 | 1 mg/ml |
| Chemical compound, drug |
poly-L-Lysine | Sigma-Aldrich | Cat. #: P8920 | pure |
| Chemical compound, drug |
Phalloidin Alexa350 | Thermo Fisher Scientific |
Cat. #: A22281 | IF (1:40) |
| Chemical compound, drug |
PureCol | Advanced BioMatrix | Cat. #: 5005–100 ML | |
| Chemical compound, drug |
Streptavidin Cy3 | Jackson Immuno Research |
Cat. #: 016-160-084; RRID: AB_2337244 |
FACS (1:400) |
| Chemical compound, drug |
Streptavidin FITC | Thermo Fisher Scientific |
Cat. #: SA10002 | FACS (1:200) |
| Chemical compound, drug |
Streptavidin PerCP-Cy5.5 | BD Pharmingen | Cat. #: 551419 | FACS (1:200) |
| Software, algorithm | ImageJ | National Institute of Health |
RRID:SCR_003070 | |
| Software, algorithm | ImSpector Pro | LaVision BioTec GmbH |
||
| Software, algorithm | Photoshop | Adobe | RRID:SCR_014199 | |
| Software, algorithm | Slidebook 6.0.8 Software | Intelligant Imaging Innovations |
RRID:SCR_014300 | |
| Software, algorithm | VirtualDub | GNU General Public License (GPL) |
||
| Other | Glass Bottom Microwell Dishes |
MatTek Corporation | Cat. #: P35G-1.5–20 C | |
| Other | Ibidi flow chambers µ-Slide I 0.8 Luer IbiTreat |
Ibidi | Cat. #: 80196 |