Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Gallus gallus domesticus) |
Chicken fertilized eggs |
Granja Gibert | ||
| Cell line (human) |
HEK293 | ATCC | RRID:CVCL_0045 | |
| Transfected construct (empty) |
pCAGGS:_ires_ GFP |
(Megason and McMahon, 2002) | ||
| Transfected construct (Rattus norvegicus) |
pCAGGS:ASCL1 | Other | Gift from François Guillemot, Francis Crick Institute, London, UK |
|
| Transfected construct (Rattus
norvegicus) |
pCAGGS:ASCL1-t- E47_ires_GFP |
(Geoffroy et al., 2009) | ||
| Transfected construct (Rattus norvegicus) | pCAGGS:ASCL1-t- ASCL1_ires_GFP |
This paper | See Materials and methods |
|
| Transfected construct (Mus
musculus) |
pCAGGS:ATOH1_ ires_GFP |
This paper | See Materials and methods |
|
| Transfected construct (Mus
musculus) |
pCAGGS:E47 | (Holmberg et al., 2008) | ||
| Transfected construct (Mus musculus) |
pCAGGS:E47bm_ ires_GFP |
This paper | See Materials and methods |
|
| Transfected construct (Rattus
norvegicus) |
pCAGGS:HA- ASCL1 |
(Alvarez-Rodríguez and Pons, 2009) | ||
| Transfected construct (Rattus norvegicus) |
pCAGGS:HA- NGN1 |
This paper | See Materials and methods |
|
| Transfected construct (Mus musculus) |
pCAGGS:ID2_ires _GFP |
This paper | See Materials and methods |
|
| Transfected construct (Rattus norvegicus) |
pCAGGS:NGN1 | Other | Gift from François Guillemot, Francis Crick Institute, London, UK |
|
| Transfected construct (Rattus norvegicus) |
pCAGGS:NGN1-t- E47_ires_GFP |
This paper | See Materials and methods |
|
| Transfected construct (Rattus norvegicus) |
pCAGGS:NGN1-t- NGN1_ires_GFP |
This paper | See Materials and methods |
|
| Transfected construct (Rattus norvegicus) |
pCAGGS:NGN2 | Other | Gift from François Guillemot, Francis Crick Institute, London, UK |
|
| Transfected construct (Rattus norvegicus) |
pCAGGS:SMAD5- SD_ires_GFP |
(Le Dréau et al., 2012) | ||
| Transfected construct (Mus musculus) |
pCAGGS:TCF12 | (Holmberg et al., 2008) | ||
| Transfected construct (Mus musculus) |
pCMV2:Flag- E47-RFP |
(Mehmood et al., 2009) | ||
| Transfected construct (Mus musculus) |
pCMV2:Flag- E47Δnls-RFP |
(Mehmood et al., 2009) | ||
| Transfected construct (human) |
pCS2:H2B-GFP | (Le Dréau et al., 2014) | ||
| Transfected construct (Mus musculus) |
pCS2:Somitabun | (Beck et al., 2001) | ||
| Transfected construct (Mus musculus) |
pMiW:myc- NGN1 |
(Gowan et al., 2001) | ||
| Transfected construct (Mus musculus) |
pMiW:myc- NGN1-AQ |
(Gowan et al., 2001) | ||
| Transfected construct (empty) |
pSuper | Oligoengine | ||
| Transfected construct (empty) |
pShin | (Kojima et al., 2004) | ||
| Transfected construct (Gallus gallus domesticus) |
sh-Bmp7 | (Le Dréau et al., 2012) | ||
| Transfected construct (Gallus gallus domesticus) |
sh-Smad1 | (Le Dréau et al., 2012) | ||
| Transfected construct (Gallus gallus domesticus) |
sh-Smad5 | (Le Dréau et al., 2012) | ||
| Transfected construct (Gallus gallus domesticus) |
sh-Id2 | This paper | See Materials and methods |
|
| Transfected construct (Mus musculus) |
pTubb3:luc | This paper | See Materials and methods |
|
| Transfected construct (Mus musculus) |
pId2(backbone): luc |
(Kurooka et al., 2012) | ||
| Transfected construct (Mus musculus) |
pId2(Full):luc | (Kurooka et al., 2012) | ||
| Transfected construct (Mus musculus) |
pId2(BREonly): luc |
(Kurooka et al., 2012) | ||
| Transfected construct (Mus musculus) |
pId2(BREmut): luc |
(Kurooka et al., 2012) | ||
| Transfected construct (Gallus gallus domesticus) |
pSox2:luc | (Saade et al., 2013) | ||
| Transfected construct (artificial) |
pKE7:luc | (Akazawa et al., 1995) | ||
| Transfected construct (Mus musculus) |
pNeuroD:luc | Other | Gift from François Guillemot, Francis Crick Institute, London, UK |
|
| Transfected construct (Mus musculus) |
pNeuroDmut: luc |
This paper | See Materials and methods |
|
| Transfected construct (Mus musculus) |
pDll1-M:luc | (Castro et al., 2006) | ||
| Transfected construct (Mus musculus) |
pDll1-N:luc | (Castro et al., 2006) | ||
| Transfected construct (Gallus gallus domesticus) |
pSox2:eGFP | (Saade et al., 2013) | ||
| Transfected construct (Mus musculus) |
pTis21:RFP | (Saade et al., 2013) | ||
| Biological sample (Gallus gallus domesticus) |
developing spinal cord (HH14-HH24) |
|||
| Biological sample (Gallus gallus domesticus) |
developing telencephalon (E3-E5) |
|||
| Antibody | anti-Ascl1 (mouse) |
BD Pharmingen |
cat #556604; RRID:AB_396479 |
|
| Antibody | Anti-Chx10 (guinea pig) |
Other | Gift from Sam Pfaff, The Salk Institute for Biological Studies, La Jolla, CA, USA; 4% PFA fixation; dilution 1/10,000 |
|
| Antibody | Anti-E2A (rabbit) |
Santa Cruz | cat #sc-349; RRID:AB_675504 |
|
| Antibody | Anti-En-1 (mouse) |
Other | Gift from Alexandra Joyner, Sloan Kettering Institute, New York, NY, USA; 4% PFA fixation; dilution 1/100 |
|
| Antibody | Anti-Evx1 (mouse) |
DSHB | cat #99.1-3A2; RRID:AB_528231 |
|
| Antibody | Anti-Gata3 (mouse) |
Santa Cruz | cat #sc-268; RRID:AB_2108591 |
|
| Antibody | Anti-HA (rabbit) |
Abcam | cat #ab20084 RRID:AB_445319 |
|
| Aantibody | Anti-HuC/D (mouse) |
Life Technologies |
cat #A-21271; RRID:AB_221448 |
|
| Antibody | Anti-ID2 (mouse) |
Abcam/Thermo Fisher Scientific |
cat #ab166708; RRID: AB_2538566 |
|
| Antibody | Anti-ID2 (rabbit) |
Calbio Reagents | cat #M213; RRID: AB_1151771 |
|
| Antibody | Anti-Isl1 (mouse) |
DSHB | cat #402D6; RRID: AB_528315 |
|
| Antibody | Anti-Lbx1 (guinea pig) |
Other | Gift from Thomas Müller and Carmen Birchmeier, Max Delbrück Center for Molecular Medicine, Berlin, Germany; 4% PFA fixation; dilution 1/10,000 |
|
| Antibody | Anti-Lhx1/5 (mouse) |
DSHB | cat #4F2; RRID: AB_2314743 |
|
| Antibody | Anti-Lhx2/9 (rabbit) |
Other | Gift from Thomas Jessell, Zuckerman Institute, Departments of Neuroscience, and Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA; 4% PFA fixation; dilution 1/5,000 |
|
| Antibody | Anti-Lmx1b (mouse) |
DSHB | cat #50.5A5; RRID:AB_2136850 |
|
| Antibody | Anti-Neurogenin-1 (rabbit) |
Millipore | cat #AB15616; RRID:AB_838216 |
|
| Antibody | Anti-Nkx6.1 (mouse) |
DSHB | cat #F55A10; RRID:AB_532378 |
|
| Antibody | Anti-Pax2 (rabbit) |
Zymed | cat #71–6000; RRID:AB_2533990 |
|
| Antibody | Anti-Pax6 (mouse) |
DSHB | Cat#pax6; RRID:AB_528427 |
|
| Antibody | Anti-Pax6 (rabbit) |
Covance/Biolegend | cat #901301; RRID:AB_2565003 |
|
| Antibody | Anti-pH3 (rat) |
Sigma Aldrich | cat #H9908; RRID:AB_260096 |
|
| Antibody | Anti-RFP (rabbit) |
(Herrera et al., 2014) | ||
| Antibody | Anti-Sox2 (rabbit) |
Abcam | cat #ab97959; RRID:AB_2341193 |
|
| Antibody | Anti-Trb1 (rabbit) |
Abcam | cat #ab31940; RRID:AB_2200219 |
|
| Antibody | Anti-Tbr2 (rabbit) |
Abcam | cat #ab23345; RRID:AB_778267 |
|
| Antibody | Anti-Tlx3 (guinea pig) |
Other | Gift from Thomas Müller and Carmen Birchmeier, Max Delbrück Center for Molecular Medicine, Berlin, Germany; 4% PFA fixation; dilution 1/10,000 |
|
| Antibody | Anti-Tubulin, beta (mouse) |
Millipore | cat #MAB3408; RRID:AB_94650 |
|
| Sequence- based reagent |
Primers for ChIP of the pKE7 promoter region: |
This study | #FW:CAggTgg CAggCAgg; #REV: AAgCAgAACg Tgggg |
|
| Sequence- based reagent |
Primers for ChIP of the pNeuroD promoter region: |
This study | #FW:CTggCTTCTAA TCTCCATCAC; #REV:gTTTCCAg ATgAggTCgT |
|
| Sequence- based reagent |
Primers for ChIP of the Luciferase ORF: |
This study | #FW:gAAgACgC CAAAAACATA; #REV:CgTATCTCT TCATAgCCTTA |
|
| Commercial assay or kit |
CellTrace Violet Cell Proliferation Kit |
Thermo Fisher Scientific |
Cat #C34557 | |
| Software, algorithm |
Adobe Photoshop CS5 |
Adobe | RRID:SCR_014199 | |
| Software, algorithm |
FlowJo | TreeStar Inc | RRID:SCR_000410 | |
| Software, algorithm |
ImageJ | (Schneider et al., 2012) | RRID: SCR_003070 | http://imagej.net/Welcome |
| Software, algorithm |
Leica LAS AF Image Acquisition |
Leica Microsystems |
RRID:SCR_013673 |