(
A) Scatter plots comparing the mean fraction of CG methylation in a 5 kb window immediately 3’ of the TSS for protein-coding genes for each of the peripheral EC biological replicates. (
B) Scatter plots comparing the mean fraction of CG methylation in a 5 kb window immediately 3’ of the TSS for protein-coding genes from liver versus lung, liver versus kidney, and lung versus kidney. Colored symbols indicate transcripts with FDR < 0.05 and enrichment >2 fold for the indicated tissue comparison. These plots show a positive correlation between EC-tissue-specific gene expression and tissue-specific hypo-methylation. (
C) Barplot indicating the distribution of UMRs, LMRs, and DMRs in relation to gene TSSs. (
D) Heatmap indicating percentage of overlap among UMRs and LMRs. There is more overlap between UMRs of different EC subtypes than between LMRs. (
E) Gene ontology categories (
McLean et al., 2010) related to TFs are strongly enriched for the genes that overlap DMVs shared by all four EC subtypes.