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. Author manuscript; available in PMC: 2019 Oct 1.

Published in final edited form as: Am J Transplant. 2018 Apr 17;18(10):2544–2558. doi: 10.1111/ajt.14718

(A–D) MDSCs infiltrating in LPC were detected as shown in Figure S5 and the Materials and Methods. The scatter plots show the percentages of total MDSCs (A), M-MDSCs (B), PMN-MDSCs (C), and e-MDSCs (D) among CD45⁺ mononuclear cells of LPC during the pre-transplant periods (before Tx, n = 8, circles), within 2 months (period I, n = 10, squares), 2 months to 1 year (period II, n =22, triangles), and more than 1 year (period III, n = 3, reverse triangles) after ITx. Each dot shows individual data of MDSCs, and horizontal lines show the mean percentage ± SEM. Statistical significances are indicated in the graphs (Mann-Whitney U test). (E) The histograms show expressions of CCR1, CCR2, CCR3, and CXCR2 on M-MDSCs (blue), PMN-MDSCs (red), e-MDSCs (orange) and internal negative (light green) and positive (dark green) controls, which are expected not to express and to express each chemokine receptors, respectively. Similar results were obtained from 5 to 10 different recipients. (F) mRNAs were extracted from pre-transplant grafts (black bar, n = 3), intestinal grafts at 3 months (striped bar, n =3), and those at 6 months (gray bar, n = 2) after ITx and were analyzed using nanoStrings^® system. Bar graphs show the mean normalized counts of mRNA ± SEM for indicated chemokine ligands. The statistical p values were calculated using a one-way ANOVA with Bonferroni post hoc tests and are indicated in the graphs (* p < 0.05).

(A–D) MDSCs infiltrating in LPC were detected as shown in Figure S5 and the Materials and Methods. The scatter plots show the percentages of total MDSCs (A), M-MDSCs (B), PMN-MDSCs (C), and e-MDSCs (D) among CD45⁺ mononuclear cells of LPC during the pre-transplant periods (before Tx, n = 8, circles), within 2 months (period I, n = 10, squares), 2 months to 1 year (period II, n =22, triangles), and more than 1 year (period III, n = 3, reverse triangles) after ITx. Each dot shows individual data of MDSCs, and horizontal lines show the mean percentage ± SEM. Statistical significances are indicated in the graphs (Mann-Whitney U test). (E) The histograms show expressions of CCR1, CCR2, CCR3, and CXCR2 on M-MDSCs (blue), PMN-MDSCs (red), e-MDSCs (orange) and internal negative (light green) and positive (dark green) controls, which are expected not to express and to express each chemokine receptors, respectively. Similar results were obtained from 5 to 10 different recipients. (F) mRNAs were extracted from pre-transplant grafts (black bar, n = 3), intestinal grafts at 3 months (striped bar, n =3), and those at 6 months (gray bar, n = 2) after ITx and were analyzed using nanoStrings^® system. Bar graphs show the mean normalized counts of mRNA ± SEM for indicated chemokine ligands. The statistical p values were calculated using a one-way ANOVA with Bonferroni post hoc tests and are indicated in the graphs (* p < 0.05).