Fig 2.

Reconstitution of the CD8 T cell compartment in Prf^−/− mice after adoptive transfer of RV-PRF–transduced Prf^−/− CD8 T cells. A, Engraftment was measured based on GFP expression. Both cohorts show similar engraftment levels without a significant difference. B, Cytotoxicity assay of reconstituted Prf^−/− or WT mice with P815 cells. Data are shown as means ± SDs of all investigated animals. Two-way ANOVA was used to calculate statistically significant differences between the WT and untreated groups, as well as the treated versus untreated groups. There is no significant difference between both interventional groups (wt CD8 vs RV-PRF-CD8). Asterisks indicate a P value of less than .05 between the treated versus untreated groups. C,In vitro IFN-γ production measured in supernatants after coincubating splenic CD8 T cells with P815 cells. Left to right, Prf^−/− mice, Prf^−/− mice transplanted with uncorrected Prf^−/− CD8 T cells, WT mice, Prf^−/− mice receiving adoptive transfer of WT CD8 T cells, and Prf^−/− mice receiving RV-PRF–transduced Prf^−/− CD8 T cells. Symbols in Fig 2, A and C, represent individual mice in each treatment group. The horizontal bar represents the median, and whiskers mark the interquartile range.