Fig E2.

Increased monocyte membrane TNF expression and adalimumab binding predicted therapeutic response. A, Correlation between the change in the percentage of Treg in vitro stimulated by adalimumab and baseline monocyte membrane TNF expression in patients before adalimumab treatment (n = 19). B, Membrane TNF expression on CD14⁺ monocytes isolated before adalimumab treatment from responders (n = 14) and nonresponders to therapy (n = 5). C, Monocyte membrane TNF expression at baseline from patients who responded to adalimumab therapy (n = 14), divided according to concurrent methotrexate therapy. D,In vitro effect of methotrexate on monocyte membrane TNF expression in PBMCs from untreated patients with RA (n = 12). E, ROC-curve analysis of the utility of baseline membrane TNF expression before therapy to predict response to adalimumab (n = 19). F, Adalimumab binding to monocytes from patients before treatment divided according to their clinical response to adalimumab (n = 18). G, ROC-curve analysis of the percentage binding of adalimumab to monocytes to predict response to adalimumab (n = 18). H, Monocyte membrane expression after culture with adalimumab or etanercept using PBMCs taken before adalimumab therapy, divided according to subsequent response (n = 14) or not (n = 5) to adalimumab. ROC, Receiver-operating characteristic. *P < .05; **P < .01; ***P < .001 by paired t test or Mann-Whitney test.