Figure 4.

With Training Activity in Layer 2/3 Decreases and Increases in Layer 5 PT Neurons

(A) Top: average turn response of a layer 2/3 neuron during a contraversive turn (left) during the first 4 days of training (early, pale blue, n = 804 turns) and the last 4 days of training (late, dark blue, n = 818 turns); and the average turn responses of another neuron during ipsiversive turns (right) early (pale red, n = 819 turns) and late (dark red, n = 873 turns) in training. Bottom: average turning speed traces corresponding to the data shown in the top panels. Shading indicates SEM over turns.

(B) Average layer 2/3 responses during contraversive (blue) and ipsiversive (red) turns early (days 1 to 4) and late (days 5 to 8) in training. Responses during contraversive turns decrease with training. Error bars indicate SEM over neurons (n = 1,154 neurons). ^∗p < 0.05, ^∗∗∗p < 10⁻³, n.s., not significant; paired Student’s t test.

(C) Average absolute Pearson’s correlation coefficient of layer 2/3 activity and turning velocity early (days 1 to 4) and late (days 5 to 8) in training. n.s., not significant; paired Student’s t test.

(D) As in (A), but for two layer 5 PT neurons (number of early contraversive turns: n = 592; late contraversive turns: n = 898; early ipsiversive turns: n = 590 turns; late ipsiversive turns: n = 899).

(E) As in (B), but for layer 5 PT neurons (n = 560 neurons). ^∗∗p < 0.01, ^∗∗∗p < 10⁻³, n.s., not significant; paired Student’s t test.

(F) As in (C), but for layer 5 PT neurons. ^∗∗∗p < 10⁻³; paired Student’s t test.