REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Chicken Anti-GFP	ThermoFisher	RRID: AB_2534023
Anti-NeuN	Millipore	RRID: AB_2298772
Alexa Fluor 488 Donkey Anti-Chicken IgY	Jackson ImmunoResearch	RRID: AB_2340375
Alexa Fluor 568 donkey anti - rabbit IgG	ThermoFisher	RRID: AB_2534017
Alexa Fluor 647 donkey anti - mouse IgG	ThermoFisher	RRID: AB_162542
Bacterial and Virus Strains
AAV2/1-EF1a-GCaMP6f-WPRE	FMI Vector Core	N/A
AAV2/1-EF1a-DiO-GCaMP6f-WPRE	FMI Vector Core	N/A
Chemicals, Peptides, and Recombinant Proteins
Isoflurane (Attane)	Provet	CAS 26221-73-3
Fentanyl	Actavis	CAS 990-73-8
Midazolam (Dormicum)	Roche	CAS 59467-96-8
Medetomidine (Dormitor)	Orion	CAS 86347-14-0
Flumazenil (Anexate)	Roche	CAS 78755-81-4
Atipamezole (Antisedan)	Orion Pharma	CAS 104054-27-5
Histoacryl	B. Braun	CAS 6606-65-1
Dental cement (Paladur)	Heraeus Kulzer	CAS 9066-86-8
Ibotenic acid	Sigma	CAS 2552-55-8
Experimental Models: Organisms/Strains
Mouse: C57BL6/J	Charles River Laboratories	N/A
Mouse: Tg(Tlx3-Cre)PL58Gsat	MMRRC	RRID: MMRRC_036670-UCD
Mouse: Tg(Sim1-Cre)KJ18Gsat	Gerfen et al., 2013	RRID: MMRRC_031742-UCD
Mouse: Slc32a1tm2(cre)Lowl/J	Vong et al., 2011	RRID: IMSR_JAX:016962
Mouse: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J	Madisen et al., 2010	RRID: IMSR_JAX:007914
Mouse: B6.Cg-Tg(Slc32a1-COP4∗H134R/EYFP)8Gfng/J	The Jackson Laboratory	RRID: IMSR_JAX:014548
Software and Algorithms
LabVIEW	National Instruments	RRID: SCR_014325
Iris: 2 photon scanning software	N/A	https://sourceforge.net/projects/iris-scanning/
MATLAB	The MathWorks	RRID: SCR_001622
Calliope: Image processing software	N/A	https://svn.code.sf.net/p/iris-scanning/calliope/
Other
Titanium Headplate	Leinweber et al., 2017	Custom
Virtual reality setup	Leinweber et al., 2017	Custom