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. 2018 Sep 6;8:13310. doi: 10.1038/s41598-018-31529-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) PCR of DNA extracted from oral and skin lesions of newborn lambs. Lane 1 gingiva, Lane 2 palate, Lane 3 lip, lanes 4–6 healthy, non-infected lambs, Lane C+ positive control (BPV-2 and -13 E5-expressing urothelial cancer samples), Lane NTC: no template control. (B) RT-PCR: Lane 1 gingiva, Lane 2 palate, Lane 3 lip, Lane 4 spleen, Lane 5 kidney, Lane 6 liver, Lanes 7–9 healthy, non-infected lambs, Lane C+ positive control (BPV-2 and -13 E5-expressing urothelial cancer cells), Lane NTC- no template control. Sequencing results of both DNA and cDNA amplicons. Alignment of the sequences detects a 100% identity with E5 bovine papillomavirus type 2 (Sequence ID: M20219.1) in (C) and type 13 (Sequence ID: JQ798171.1) in (D).