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. 2018 Sep 6;8:13373. doi: 10.1038/s41598-018-31075-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Line plots showing TSC1 (a) and TSC2 (b) gene expression over time, using data from the Human Brain Transcriptome (http://hbatlas.org/). NCX = neocortex, MD = medial dorsal thalamus, CBC = cerebellar cortex, HIP = hippocampus, STR = striatum and AMY = amygdala. ‘Y-axis shows relative gene expression as Log2 signal intensity assessed from hybridization of cDNA generated from extracted RNA to Affymetrix Human Exon microarrays as detailed in Kang et al., Nature 2011. ‘Period’ refers to periods of human development and adulthood defined in that original paper and chosen to emphasize the timing and progression of major neurodevelopmental events in the Cerebral Cortex.’ X-axis shows post-conception age in days; the solid vertical line indicates the transition between pre-natal and post-natal periods.