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. 2018 Sep 6;8:13344. doi: 10.1038/s41598-018-30813-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genotyping of clinical P. aeruginosa isolates that displayed a weakened biofilm formation. (a) Mutations in rhlI gene that resulted in changes in amino acid residues in biofilm-deficient isolates. (b) Mutation in lasR genes in two isolates, bj13 and bj14. The red box show the changes in the 180^th nucleotide in lasR leading to LasRW60* mutation. All sequencing results were compared to reference PAO1 genome sequences. (c) PFGE pattern of clinical isolates. Genomic DNA extracted from the isolates were digested with SpeI. PAO1 genomic DNA digested with SpeI was used as a control.