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. 2018 Sep 6;8:13344. doi: 10.1038/s41598-018-30813-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Difference in rhl-related gene expression in P. aeruginosa isolates. (a) C4HSL levels assessed by a reporter assay. The supernatants of P. aeruginosa culture grown for 18 hours were assayed for C4HSL levels. (b–d) The relative mRNA levels of rhlR,rhlA and pqsA in P. aeruginosa. The total RNAs from P. aeruginosa at the stationary phase were used for real time qRT-PCR analysis. Relative mRNA levels were obtained by normalizing the level of rhlR, rhlA and pqsA to that of the rplS. The representative data from the strains with backgrounds of PAO1 (b), bj13 (c), or bj14 (d) were reported as mean ± SD of quadruplicates in an experiment. Experiments were repeated for three times. ****P < 0.0001; P > 0.05, ns (no significance). P values were obtained by one way ANOVA with Bonferroni test.