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. 2018 Sep 6;8:13313. doi: 10.1038/s41598-018-31275-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of different media on bio-volume and thickness of bacteria and EPS. The images of the biofilms formed in 6 different media (1. 0% sucrose +0% glucose, 2. 0.5% sucrose, 3. 1% sucrose, 4. 0.5% glucose, 5. 1% glucose, and 6. 0.5% sucrose +0.5% glucose) using confocal laser scanning microscopy, and (a) bio-volume and (b) thickness of bacteria (green) and EPS (red) of the biofilms were quantified by COMSTAT software. Data represent the mean and standard deviation of 3 independent experiments. Values followed by the same superscript are not significantly different (p > 0.05).