FIGURE 7.
1-MT affects the motility of P. syringae. (A) RNA extraction was performed from bacteria extracted from treated and untreated plants 72 hpi. Relative expression of fliC during bacterial growth was analyzed. The recA gene was used as an endogenous reference gene. (B) Swimming diameter was determined 72 hpi in KB agar plates (containing 50% KB and 0.25% agar) with or without 1-MT at 1.5 or 5 mM where 5 μl of 5 × 105 cfu mL-1 bacterial suspension was inoculated. The plates were incubated at 28°C and five plates by treatment were measured. The results represent the average of three independent experiments ± SE. Different letters indicate statistically significant differences (p < 0.05, least-significant difference test).