Fig. 4.

P_JEx-mediated transcription in non-enteric proteobacteria. a Fluorescence of P. putida KT2440, S. (Ensifer) meliloti Rm1021 and C. crescentus NA1000 cultures expressing RFP under the control of EilR-regulated P_JEx promoters, either in their repressed state, or induced by 1 µM CV. The TetR-regulated P_LtetO-1 promoter¹¹, induced with 100 nM anhydrotetracycline, was included for comparison. b Fluorescence of cultures expressing RFP from three P_JEx promoters induced by increasing CV concentrations. Data represent the averages of plate reader measurements from two (P. putida) or three (S. meliloti, C. crescentus) independently grown stationary phase cultures, with the standard deviation indicated by error bars (curves) or in parentheses (table). Fluorescence values are in arbitrary units and cannot be compared directly across different hosts because different instruments were used for measurements