Table 3.
Parameters used in the LC-MS analysis.
| Compound | Retention time (minutes) | Ionisation mode | SRM transition | Collision energy |
|---|---|---|---|---|
| Phenacetin | 4.3 | Positive | 180 > 110 | 24 |
| Paracetamol | 3.1 | Positive | 152 > 110 | 24 |
| Tolbutamide | 1.8 | Negative | 269 > 170 | 20 |
| 4-Hydroxytolbutamide | 1.1 | Negative | 285 > 186 | 20 |
| Carboxytolbutamide | 1.2 | Negative | 299 > 200 | 19 |
| Diclofenac | nd | nd | nd | nd |
| 4-Hydroxydiclofenac | 4.9 | Positive | 312 > 230 | 24 |
| Amitriptyline | 4.7 | Positive | 278 > 91 | 32 |
| Nortriptyline | 4.6 | Positive | 264 > 91 | 32 |
| Clomipramine | 4.2 | Positive | 315 > 86 | 24 |
| Norclomipramine | 4.1 | Positive | 301 > 72 | 24 |
| Dextromethorphan | 3.8 | Positive | 272 > 171 | 37 |
| Dextrorphan | 3.4 | Positive | 258 > 157 | 38 |
| 3-Methoxymorphinan | 3.7 | Positive | 258 > 215 | 20 |
| Nifedipine | 5.4 | Positive | 329 > 284* | 24 |
| Oxidised nifedipine | 5.1 | Positive | 345 > 284 | 24 |
Rows group parent compound (top) and corresponding metabolites. nd = not detected, *parent ion selected results from loss of water from the protonated molecular ion.