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. 2018 Sep 6;8:13381. doi: 10.1038/s41598-018-31249-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Confocal and scanning electron microscopy (SEM) analyses of Salmonella enterica subsp. enterica serotype Typhimurium after challenge with five times the minimal bactericidal concentraiton of small molecules (5X MBC of SMs) for 3 hrs. (A–O) Confocal microscopy: (A–E) S. Typhimurium cell membrane stained with FM4-64; (F–J) S. Typhimurium nucleic acids stained with SYTO9; (K–O) Merged pictures of the FM4-64 and SYTO9 staining. (P–S) SEM: (P) 1% dimethyl sulfoxide (DMSO) treated Salmonella; (Q) SM1 treated Salmonella; (R) SM4 treated Salmonella; (S) SM5 treated Salmonella; Bar: 1 μm.