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. 2018 Sep 6;8:13350. doi: 10.1038/s41598-018-31625-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sedimentation velocity, ls-g*(s) and c(s) analysis for pooled saliva. The plots show the sedimentation coefficient distributions for unstimulated (US) and stimulated (SS) samples. Saliva fingerprinting is based on the sedimentation coefficient distribution, directly derived by fitting the Lamm equation in SEDFIT^20,25,46. The plots shows typical sedimentation coefficient profiles for the sedimentation species present in saliva analysed by two methods, c(s) (top) and ls-g*(s) (bottom). Peaks are identified based on their molecular weight (directly related to the sedimentation coefficient) and their relative concentration. Estimations have been confirmed with individual runs for porcine α-amylase and bovine submaxillary mucin. The US and SS distributions are shown in blue and red respectively. Rotor speed: 40 000 rpm (120 000 g), 20.0 °C.