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. 2018 Apr 22;3(5):1193–1201. doi: 10.1016/j.ekir.2018.04.009

Figure 2.

Figure 2

Proteomic identification of APOC2 Lys41Thr in glomerular amyloid deposits. Congo red−positive glomeruli were microdissected and analyzed using liquid chromatography with mass spectrometry (LC-MS/MS), as described in the Methods. (a) Protein identification report consisting of representative dissections from all 5 cases is shown. Numbers in green boxes represent number of MS/MS matches to the respective protein. Universal amyloid tissue marker proteins (apolipoprotein E [APOE], APOA4, and serum amyloid protein [SAP]) are shown in gold stars. Type-specific marker (APOC2) is shown in the blue star. (b) Sequence coverage map of wild-type APOC2, from the patient 1 specimen, showing that the entire protein is deposited. Amino acids highlighted in bold letters over yellow background were detected in the deposit. The first 22 amino acids correspond to the signal peptide. (c) MS/MS spectrum corresponding to the APOC2 p.Lys41Thr mutant peptide is shown. Blue arrow shows the location of the mutation in the detected peptide. (d) Peptides corresponding to both mutant and wild-type forms of APOC2 were detected in the deposit. Distribution of normalized MS/MS matches to the corresponding APOC2 forms are shown.