Figure 3.

The model predicts the relation of protein allocation fractions and the growth rate when one flux capacity (k₁, k₀, or 1/ϕ_m) is reduced by the stress. Insets present the decrease of growth rate with flux capacity limitation, in which units of μ, k₁, k₀, and ϕ_m are doublings/hour (dbls/h), h⁻¹, h⁻¹, and 1, respectively. ϕ_R, ϕ_P1, ϕ_P2, and ϕ_P3 indicate proteome fractions of ribosome-affiliated proteins (R class), AA-supply-required proteins (P₁ class), chaperone-like proteins (P₂ class), and protease-like proteins (P₃ class), respectively. The experimental data for ϕ_P2 (circles) and ϕ_P3 (triangles) are obtained with the classification of Proteomaps (49); those in (A) are based on the proteomic data of (22, 28, 29, 30), and those in (B) are based on (22). Common parameters: ϕ^∗ = 0.55, ϕ₀ = 0.066, k₃ = 0.3 h⁻¹. (A) AA supply stress is shown by k₁ decreasing. Parameters: k₀ = 6 h⁻¹ and ϕ_m = 0.0061. (B) Translational stress is displayed by k₀ decreasing (k₁ > k₃). Parameters: k₁ = 4.5 h⁻¹ and ϕ_m = 0.0061. (C) Protein maturation stress is reflected by ϕ_m increasing (k₁ > k₃). Parameters: k₀ = 6 h⁻¹ and k₁ = 4.5 h⁻¹. To see this figure in color, go online.