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. 2018 Jun 27;26(9):2267–2281. doi: 10.1016/j.ymthe.2018.06.019

Figure 2.

Figure 2

LINC00470 Functions as a Sponge for miR-101 to Facilitate ELFN2 Expression

(A) Luciferase reporter assays were performed to detect the relationship between miR-101 and ELFN2. The data shown are the means ± SEM of six replicates and are representative of three independent experiments. *p < 0.05. (B) qRT-PCR was performed to detect the expression of ELFN2 in GBM cells after transfection with miR-101 mimics. The data are presented as the means ± SEM of three independent experiments. *p < 0.05. (C) Western blotting was performed to detect the expression of ELFN2 in GBM cells after transfection with miR-101 mimics. (D) qRT-PCR was performed to detect the expression of miR-101 in GBM cells after transfection with pcDNA3.1-LINC00470. The data are presented as the means ± SEM of three independent experiments. *p < 0.05. (E) qRT-PCR was performed to detect the expression of LINC00470 in GBM cells after transfection with miR-101. The data are presented as the means ± SEM of three independent experiments. *p < 0.05; **p < 0.01. (F) Western blotting was performed to detect the expression of ELFN2 in GBM cells after transfection with pcDNA3.1-LINC00470. (G) qRT-PCR was performed to detect the expression of ELFN2 in GBM cells after transfection with miR-101 and LINC00470. The data are presented as the means ± SEM of three independent experiments. *p < 0.05. (H) Western blotting was performed to detect the expression of ELFN2 in GBM cells after transfection with miR-101 and LINC00470.