Figure 1.

Experimental treatment scheme. To evaluate the effect of P2Et as prophylactic therapy in both murine models, mice received the following treatment scheme: PBS injection → tumor cells → PBS injection (PBS/PBS), PBS injection → tumor cells → P2Et treatment (PBS/P2Et), and P2Et treatment → tumor cells → P2Et treatment (P2Et/P2Et). The pretreatment was administered via i.p two times per week for 10 days, the tumor was later established by injection of B16F10 or 4T1 cells, and finally, 3 days after tumor cell injection, the post-treatment was s.c. administered around the tumor two times per week for 21 and 32 days, respectively.