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Wolbachia titers in dsVg-microinjected L. striatellus. Second instar nymphs were microinjected with Vg dsRNA or dsGFP as a control. After dsRNA injection, 2-day-old emerged female insects were selected to detect Vg expression in the whole body (A). Wolbachia density significantly decreased in the dsVg-injected ovaries of 2-day-old adult insects (B) and did not differ in the whole body (C). Bars show the average fold change per experiment ± SD. (∗∗∗P < 0.001).
