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. 2018 Aug 30;12:283. doi: 10.3389/fncel.2018.00283

Figure 2.

Figure 2

Als effectively rescued c-Myc from the MPP+-induced decline via Wnt signaling. (A) Cells transfected with pcDNA3.0-β-catenin were treated with MPP+ (500 μM) for 24 h. Western blot was conducted in the Control, MPP+ and MPP++β-catenin groups. (B) Relative amounts of β-catenin/actin, c-Myc/actin and p- GSK-3β (ser9)/GSK-3β level were quantified (the means ± SEM; n = 3; *p < 0.05, **p < 0.01). (C) Cells transfected with β-catenin siRNA were treated with MPP+ (500 μM) for 24 h. Western blot was conducted in the Control siRNA, MPP+ and β-catenin siRNA groups. (D) The ratios of β-catenin/actin, c-Myc/actin and p-GSK-3β(ser9)/GSK-3β were analyzed (the means ± SEM; n = 3; *p < 0.05, **p < 0.01). (E) Cells transfected with pcDNA3.0 or pcDNA3.0-β-catenin were pretreated with Als for 24 h before MPP+ treatment. Western blot was conducted in different groups. (F) Quantification graphs of the ratios of β-catenin/actin, c-Myc/actin and p-GSK-3β (ser9)/GSK-3β (the means ± SEM; n = 3; *p < 0.05, **p < 0.01). (G) Cells transfected with control siRNA or β-catenin siRNA were pretreated with Als for 24 h before MPP+ treatment and Western blot was conducted in different groups. (H) Quantification graphs indicating the ratios of β-catenin/actin, c-Myc/actin and p-GSK-3β(ser9)/GSK-3β (the means ± SEM; n = 3; *p < 0.05, **p < 0.01).