Figure 4.

Protection of Als against the MPP⁺-induced mitochondrial fission in SH-SY5Y cells. (A) Cells were treated as described in Figure 2E. The morphology of mitochondrial was imaged using a confocal microscope (scale bar, 10 μm; Transfection efficiency, 30%). (B) Cells were treated as described in Figure 2G. Mitochondrial morphology was captured using a confocal microscope. (C,D) Quantification of the morphology of mitochondria. The ratio between the major and minor axes of an ellipse equivalent to the shape of the mitochondrial is regard as the aspect ratio of mitochondria. Twenty fields of view in a coverslip are randomly recorded to analyze the aspect ratio of mitochondria. The experiments were independently taken three times (the means ± SEM; n = 3; *p < 0.05, **p < 0.01).