Figure 10.
Comparison of Minicircle, Plasmid, and Viral-Mediated Transgene Expression
(A) Representative BLI images of animals injected with AAV, minicircle (MC), and regular plasmid in the right leg (first injection), followed by the left leg 28 days later (second injection). As expected, AAV expression is more robust compared to MC and plasmids initially. However, after repeat injection, AAV expression is not detected in the contralateral leg because of host-mediated humoral immune response. Color scale bar values are expressed as photons per second per square centimeter per steradian (p/s/cm2/sr). (B) Graphical representation of longitudinal bioluminescence imaging (BLI) after first and second injections in all 3 groups. Note that day 28 of second injection in left leg would represent day 56 of first injection in right leg in the same animal.134 Error bars, SEM.