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. 2018 Sep 7;38(5):BSR20181109. doi: 10.1042/BSR20181109

Figure 3. Stability of the inducible 2LTRZFP following sequential inductions with Dox.

Figure 3

The 2LTRZFPmCherry expression was induced by the presence of Dox twice. SupT1-Tet-On-2LTRZFP cells were initially cultured with or without Dox for 3 days. After incubation, Dox was removed from the system to allow expression to decay. (A) After the first induction, expression of 2LTRZFPmCherry was monitored for 13 days by flow cytometry. (B) After the mCherry expression diminished to the background level, the cells were again induced with Dox for 3 days and expression of mCherry was again monitored.