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. 2018 Sep 7;38(5):BSR20181109. doi: 10.1042/BSR20181109

Figure 5. Kinetics of the antiviral activity of induced 2LTRZFP against replication of the HIV-1X4-tropic strain.

Figure 5

Wild-type SupT1, and SupT1-Tet-On-2LTRZFP and SupT1-Tet-On-Aart cell lines in the presence and absence of Dox, were challenged with HIV-1NL4-3 at an MOI of 10. (A) Every 3 days, supernatants and cells were collected and p24 protein measured by ELISA and (B) at day 12, viral integration was detected using Alu-gag real-time PCR. Relative Alu-Gag level was calculated compared with GAPDH (internal control) using the comparative CT method (2−ΔΔCt). Data are represented as fold change (log10) compared with the level of SupT1 control as mean ± S.D.; n=3; ***P<0.001.