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. 2018 Sep 7;38(5):BSR20181320. doi: 10.1042/BSR20181320

Figure 3. PDI-mediated holotoxin disassembly as monitored by SPR.

Figure 3

(A) PDI or (B) heat-denatured PDI was perfused over a CT-coated SPR sensor at 30°C in PBS-T buffer containing 1 mM GSH. PDI was removed from the perfusion buffer (denoted by the asterisks) and replaced with buffer containing sequential additions of anti-PDI, anti-CTA1, and anti-CTB antibodies as indicated by the arrowheads. Before initiating the experiment at time 0, a 10-min perfusion with PBS-T was used to generate a stable baseline corresponding to the mass of the sensor-bound CT holotoxin, which was set as zero RIU.