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. 2018 Jul 5;96(9):3657–3669. doi: 10.1093/jas/sky263

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© The Author(s) 2018. Published by Oxford University Press on behalf of the American Society of Animal Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Effects of Digestarom DC (D-DC), grape seed extract (GRS), licorice extract (LIC), oak bark extract (OAK), oregano essential oil (ORE), and the plant powder mix (PLA) on gene expression of the antioxidative gene heme oxygenase 1 (HMOX1) in intestinal porcine epithelial cells (IPEC-J2). Gene expression was analyzed via RT-qPCR. Cells were preincubated with the test substances for 1 h, after which oxidative stress was induced by stimulation of the cells with 0.5 mM H₂O₂ for 1 h. The untreated cell control (CC) was set to 0 and is not shown in the figure. Cells treated with the test substances were compared with the H₂O₂-stimulated control, and results are presented as the binary logarithm (log₂) of the fold increase. Data represent the mean and SEM of five independent experiments (n = 5). No significant differences were found.