Figure 1.

Hepatocyte growth factor supplementation does not alter time to S-phase. Fresh isolates of eqSC (n = 5) were allowed to attach overnight followed by treatment with 0, 1, 5, 10, and 25 ng/mL HGF for 72 h. Cells were pulsed with EdU for 2 h prior to fixation at 24-h intervals. EdU (+) and total nuclei were enumerated. Percent EdU = EdU(+)/Hoechst 33342 (+) × 100. Means and SEMs shown. Black symbols (0 and 25 ng/mL) are different (P < 0.05) from one another at 72 h only. eqSC = equine satellite cell.