Figure 4.

Protein kinase C mediates the proliferative effects of HGF. Semi-confluent eqSC (n = 4) were treated with HGF in the presence or absence of 2 µM Gö6983 for 48 h (A). Cells were pulsed with EdU 2 h prior to fixation. Total and EdU (+) nuclei were enumerated. Isoforms of PKC expressed by eqSC were identified by RT-PCR using gene-specific primers. Amplicons were separated electrophoretically through SYBR-Safe impregnated agarose gels. (B). RNA isolates from two animals are shown per gene of interest. Molecular weight ladder ranges from 1 kb to 25 bp. Amplicon sizes are GAPDH, 150 bp, PKCα, 201 bp, PKCδ, 102 bp and PKCε, 191 bp. Percent EdU = EdU (+)/Hoechst (+) × 100. Means and SEMs shown. Means with different letters are significant at P < 0.05. eqSC = equine satellite cell; GAPDH = glyceraldehyde phosphate dehydrogenase; RT-PCR = real-time PCR.