Figure 6.

Hepatocyte growth factor inhibits eqSC differentiation. Eighty percent confluent eqSC (n = 4) were treated with HGF for 24 or 48 h followed by fixation and immunocytochemical detection of myogenin or MyHC. Representative black and white photomicrographs of myogenin expression at equal shutter speeds for CON cells receiving vehicle and eqSC treated with 25 ng/mL HGF are shown (A). Percent myogenin (+) was calculated as total myogenin/total Hoechst 33342 × 100 (B). Representative black and white images of MyHC immunostaining at 48 h (C). Percent fusion was calculated as total number nuclei within a MyHC myotube/total number nuclei × 100 (D). Scale bar = 100 µm. *Significance at P < 0.05. eqSC = equine satellite cell.