Table 1. Calculated Limit of Blank and Limit of Detection Values for Detection of α-Synuclein Aggregates with Each Extrinsic Dyea.
| ThT | diThT | pFTAA | Pico Green | |
|---|---|---|---|---|
| LoB, events·bin–1 | 2.88 × 10–3 | 1.05 × 10–3 | 1.27 × 10–3 | 4.46 × 10–2 |
| LoD, events·bin–1 | 8.28 × 10–3 | 1.71 × 10–3 | 3.35 × 10–3 | 6.11 × 10–2 |
| LoD aggregate concn, pM | 10 | 2 | 4 | 60 |
| signal-to-noise ratio | 34.5 | 57.5 | 34.9 | 1.0 |
a
The lowest concentration of aggregate that could be accurately detected with each dye was also calculated, as well as the signal-to-noise ratio.