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. 2018 Jul 20;96(9):3928–3942. doi: 10.1093/jas/sky251

Figure 1.

Figure 1.

Percent of total sugar [glucose (Glu) + xylose (Xyl)] released from barley straw. All treatments were applied at 15 mg of protein load per gram glucan of 0.5% barley straw suspension in 50 mM sodium citrate (pH = 6.0). Recombinant enzyme treatments were a mixture of 50% of rumen enzymes and 50% of the recombinant enzyme. The reaction mixture was incubated in triplicate at 39 °C for 48 h. PLY3A, pectate lyase, EC 4.2.2.2, PL3 from Pleurotus ostreatus; CBH7B, cellobiohydrolase, EC 3.2.1.91, GH7 from Phanerochaete chrysosporium; EGL12A, endoglucanase, EC 3.2.1.151, GH12, Gloeophyllum trabeum; EGL7A, endo-β-1,4-glucanase, EC 3.2.1.4, GH7 from Thielavia terrestris; PGA28A, endo-polygalacturonase, EC 3.2.1.15, GH28 from Gloeophyllum trabeum; XEG12A, xyloglucanase, EC 3.2.1.151, GH12 from Aspergillus niger; AXH62B, arabinoxylan arabinofuranosidase, EC 3.2.1.55, GH62 from Myceliophthora thermophile; AXH62A, arabinoxylan arabinofuranosidase, EC 3.2.1.55, GH62 from M. thermophile; ABF54B, alpha-arabinofuranosidase, EC 3.2.1.55, GH54 from Chaetomium thermophilum; XYL10A, 1,4-β-xylanase, EC 3.2.1.8, GH10 from Rasamsonia emersonii; XYL10C, 1,4-β-xylanase, EC 3.2.1.8, GH10 from A. niger; RME, crude mixture of rumen enzymes.