Table 1.

Chemical composition of the substrates used in in vitro experiments

	Barley straw1	Concentrate2	Diet3
DM, %	95.1	95.6	95.3
OM, % of DM	93.2	89.7	92.2
CP, % of DM	7.0	34.6	15.3
NDF, % of DM	77.7	36.0	65.2
ADF, % of DM	44.6	12.5	35.0

¹Barley straw used in batch cultures and RUSITEC studies.

²Composition (DM basis): 66.7% corn dried distillers grains with solubles, 26.6% canola meal, 4.2% calcium carbonate, 1% urea, 0.8% dicalcium phosphate, 0.5% salt, 0.17% feedlot premix, and 0.01% vitamin E.

³Diet (70% barley straw:30% of concentrate, DM basis) fed to the RUSITEC.