Table 3.

Effect of fibrolytic enzymes on DM, NDF, and ADF disappearance of barley straw after 12, 24, and 48 h of incubation in ruminal batch culture

Treatments1	Dose, µg/g	DMD, %			NDFD, %			ADFD, %
		12 h	24 h	48 h	12 h	24 h	48 h	12 h	24 h	48 h
Control	0	19.5	31.5	40.7	10.0	24.7	35.5	7.4	21.9	32.5
Viscozyme L	100	20.8	33.0	41.2	10.9	26.0	35.6	5.9	23.9	33.2
	500	20.7	33.6	41.5	11.2	26.6	36.2	8.6	22.9	34.3
EGL7A	100	21.2	33.6	41.3	11.9	26.8	36.1	7.4	24.1	33.3
	500	20.7	33.8	40.6	10.8	26.8	35.4	7.6	23.8	33.6
XYL10A	100	20.8	32.9	42.1	10.9	25.9	37.2	7.8	23.4	34.4
	500	21.1	31.7	42.3	10.9	24.5	37.0	4.9	21.4	35.4
XYL10C	100	20.4	33.2	42.7	10.5	26.3	37.7	5.9	24.1	35.5
	500	21.3	32.8	44.0	11.6	25.7	38.9	6.6	22.4	36.4
Enzyme mixture	100	21.2	34.0	41.7	11.0	27.2	36.0	5.7	24.7	33.1
	500	20.7	32.8	43.6	10.9	25.8	38.9	8.7	22.5	35.6
SEM		1.22			1.51			1.47
Contrasts P-value2
Con vs. Enz		<0.001			0.008			0.08
Con vs. Enz 100		0.002			0.01			0.12
Con vs. Enz 500		0.001			0.01			0.07
Enz 100 vs. Enz 500		0.73			0.82			0.69
Con vs. Viscozyme L		0.02			0.08			0.18
Con vs. EGL7A		0.01			0.03			0.12
Con vs. XYL10A		0.01			0.09			0.35
Con vs. XYL10C		<0.001			0.003			0.06
Con vs. enzyme mixture		<0.001			0.007			0.08

¹Viscozyme L (Novozymes Inc., Copenhagen, Denmark) = commercial crude enzyme preparation from Aspergillus aculeatus; EGL7A = endo-β-1,4-glucanase, EC 3.2.1.4, GH7 from Thielavia terrestris; XYL10A = 1,4-β-xylanase, EC 3.2.1.8, GH10 from Rasamsonia emersonii; XYL10C = 1,4-β-xylanase, EC 3.2.1.8, GH10 from Aspergillus niger; enzyme mixture = 20:20:20:20:20 mix of EGL7A, XYL10A, XYL10C, AXH62A (arabinoxylan arabinofuranosidase, EC 3.2.1.55, GH62 from Myceliophthora thermophile), and AXE1A (acetylxylan esterase, EC 3.1.1.72, CE1 from M. thermophile).

²Con = control treatment; Enz = all enzyme treatments; Enz 100 = enzyme treatments with 100 µg of protein load per gram of substrate DM; Enz 500 = enzyme treatments with 500 µg of protein load per gram of substrate DM.