Table 1.
Primer | 5ʹ → 3ʹ nucleotides sequence | Optimized concentration (μM) |
---|---|---|
F3 | GACTTCTGCTGGGTCATG | 0.4 |
B3 | GCCAACAATTCCGACACTA | 0.4 |
FIP | CCCCACTGAACCCCATCTATTGGGTCTTGGCGATTCTAGC | 2.4 |
BIP | GTTCAAGAAAGATCTGGCTGCCCCTCGTCTCTTCTTCTCCT | 2.4 |
Loop F | GCTTGATTGCCGTGAATCTC | 1.0 |
Loop B | GCTGAGAATAATCAATGCCAGG | 1.0 |