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. Author manuscript; available in PMC: 2019 Feb 20.
Published in final edited form as: Nat Methods. 2018 Aug 20;15(9):732–740. doi: 10.1038/s41592-018-0104-1

Figure 2. MEK1 inhibition is sufficient to sustain naïve pluripotency in 5i/LAF.

Figure 2.

(a) Alternative MEK inhibitors used in this study. (b) Flow cytometric analysis of the proportion of WIBR3 ΔPE OCT4GFP+ hESCs cultured as indicated. (c) Representative immunofluorescence images of UCLA4 hESCs grown in the indicated media (P8) (scale bar: 100 μm). (d) Flow cytometric analysis of the proportion of ΔPE OCT4GFP+ cells after primed-to-naive conversion of WIBR3 hESCs in 5i/LAF supplemented with the indicated MEKi.