A. IL-6r was injected intraplantar into male mice at the same time as animals received an intrathecal injection of either TNP-ATP or skepinone. Mechanical hypersensitivity was then tested at indicated time points (n = 4–6 mice per group). B. Both male and female animals that received TNP-ATP at the time of IL-6r injection had the acute response to IL-6r blocked. In male mice that received TNP-ATP, priming was blocked. C. Skepinone had no impact on the acute response to IL-6r in males or females, but attenuated hyperalgesic priming in male mice. Data are displayed as mean ± SEM. Two-way anova with Bonferroni post hoc test, * p<0.05, *** p<0.001, **** p<0.0001.