Figure 4.

Diaphragm Muscle Pathology of IV- and ICV-Treated nmd Mice Is Equally Repaired Compared to Untreated Mice

(A and C) VVG-stained cross sections of diaphragm muscles of IV- and ICV-treated males were examined at 8 weeks post-injection under 10× (A) and 40× (C) magnifications and compared with those of age-matched untreated nmd and healthy control (n = 4 for each group). (B) The area size of muscle fiber in both IV- and ICV-treated nmd mice is significantly increased compared to untreated mice (one-way ANOVA, p < 0.05). (D) The intensity of interstitial and periarterial fibrosis is significantly decreased in diaphragm muscles of IV- and ICV-treated nmd mice compared to untreated mice (one-way ANOVA, p < 0.001). Error bars represent mean ± SEM. Scale bars, 50 μm. *p < 0.05 and ***p < 0.001.