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. 2018 Sep 6;16:98. doi: 10.1186/s12915-018-0560-1

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© Smith et al. 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Alternative fluorescence (FM-AT), electron (EM-AT) and combined (FM/EM-AT) modes of array tomography. An array of serial ultrathin sections (a) (e.g., a single-ribbon coverslip produced as in Fig. 1) may be stained and imaged for multiplex fluorescence microscopy (dotted arrow 1), scanning EM (dotted arrow 2), or both (dotted arrows 1 and 3). (b–e) Schematizes possible combinations of spectral and sequential fluorescence multiplexing modes. (f, g) Schematizes (optional) array staining and image acquisition for electron microscopy