Fig. 4.

FM-AT images from varied regions of mammalian brain sampling the wide range of neuroscience FM-AT applications to date. a Synaptic anatomy of the dorsal raphe nucleus (from Fig. 2 in [102]). b Mechanistic analysis of excitatory synaptic transmission and synaptic plasticity in CA3 hippocampus (from Fig. 3 in [97], Copyright (2016), with permission from Elsevier). c Synaptic localization of MHCI proteins in mouse lateral geniculate nucleus at P7, during retinogeniculate critical period (from Fig. 4 in [98], Copyright (2009), with permission from Elsevier). d Correlated immunofluorescence/DNA-FISH images showing expansion of H4K20me3 histone modification into pericentromeric heterochromatin in Mecp2-null (GFP-) but not control (GFP+) nuclei in a mosaic Rett syndrome model mouse (from Fig. 4 in [56], Copyright (2015), with permission from Elsevier). e Human neocortex illustrating clear imaging of brightly autofluorescent lipofuscin granules (yellow) without out-of-focus flare obscuration of nearby cellular features (blue, DAPI; red, GABA; cyan, tubulin; green, neurofilament heavy chain). (Unpublished data courtesy Kristina Micheva)