Fig. 8.

Automated array construction and high-throughput fluorescence imaging. a “ArrayBot”, based on a standard ultramicrotome, combines ten computer-controlled motion axes with machine vision camera to automate the most critical steps in serial array construction and facilitate construction of large sets of contiguous single-ribbon arrays. b Robotically manipulated deer hair positions serial-section ribbon on water surface in ArrayBot trough. c “Robofluidic” AT fluorescence microscope automates staining and image acquisition across multiple single-ribbon serial-section arrays. This microscope is optimized for image acquisition speed via intense laser illumination, fast mechanics, and tight control timing. Both were developed at the Allen Institute to facilitate high-throughput, large-scale array tomography